ProSci

SGK3 Antibody

Product Code:
 
PSI-63-303
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of hSGKL-K123 (SGK3) in Jurkat and HepG2 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Western blot analysis of hSGKL-K123 (SGK3) in Jurkat and HepG2 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

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PSI-63-303-400ul400ul£626.00
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Further Information

Additional Names:
Serine/threonine-protein kinase Sgk3, Cytokine-independent survival kinase, Serum/glucocorticoid-regulated kinase 3, Serum/glucocorticoid-regulated kinase-like, SGK3, CISK, SGKL
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
SGK3, a Ser/Thr protein kinase, is similar to serum- and glucocorticoid-induced protein kinase (SGK), but this gene product is not induced by serum or glucocorticoids. Expression is induced in response to signals that activate phosphatidylinositol 3-kinase, which is also true for SGK.
Background References:
  • Friedrich, B., et al., Pflugers Arch. 445(6):693-696 (2003).
  • Embark, H.M., et al., Pflugers Arch. 445(5):601-606 (2003).
  • Brickley, D.R., et al., J. Biol. Chem. 277(45):43064-43070 (2002).
  • Gamper, N., et al., Pflugers Arch. 445(1):60-66 (2002).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SGK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-139 amino acids from the Central region of human SGK3.
NCBI Gene ID #:
100533105, 23678
NCBI Official Name:
Serine/threonine-protein kinase Sgk3
NCBI Official Symbol:
SGK3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
57 kDa
Protein Accession #:
Q96BR1
Protein GI Number:
28558163
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q96BR1
User NOte:
Optimal dilutions for each application to be determined by the researcher.