ProSci

ATP6V1F Antibody

Product Code:
 
PSI-60-349
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in Jurkat cell line lysates (35ug/lane).
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Western blot analysis in Jurkat cell line lysates (35ug/lane).

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PSI-60-349-400ul400ul£626.00
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Further Information

Additional Names:
V-type proton ATPase subunit F, V-ATPase subunit F, V-ATPase 14 kDa subunit, Vacuolar proton pump subunit F, ATP6V1F, ATP6S14, VATF
Application Note:
For WB starting dilution is: 1:1000
Background:
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein.
Background References:
  • Supino, R., et al. Ann. N. Y. Acad. Sci. 1171, 606-616 (2009) :
  • Smith, A.N., et al. J. Bioenerg. Biomembr. 40(4):371-380(2008)
  • Morel, N. Biol. Cell 95(7):453-457(2003)
  • Smith, A.N., et al. Mol. Cell 12(4):801-803(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This ATP6V1F antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-111 amino acids from the C-terminal region of human ATP6V1F.
NCBI Gene ID #:
9296
NCBI Official Name:
V-type proton ATPase subunit F
NCBI Official Symbol:
ATP6V1F
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
13 kDa
Protein Accession #:
Q16864
Protein GI Number:
126302612
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q16864
User NOte:
Optimal dilutions for each application to be determined by the researcher.