ProSci

MRPL2 Antibody

Product Code:
 
PSI-59-076
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in MCF-7 cell line lysates (35ug/lane).

Western blot analysis in MCF-7 cell line lysates (35ug/lane).

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PSI-59-076-400ul400ul£626.00
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Further Information

Additional Names:
39S ribosomal protein L2, mitochondrial, L2mt, MRP-L2, MRPL2
Application Note:
For WB starting dilution is: 1:1000
Background:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL2 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 12q.
Background References:
  • Lamesch, P., et al. Genomics 89(3):307-315(2007)
  • Mungall, A.J., et al. Nature 425(6960):805-811(2003)
  • Zhang, Z., et al. Genomics 81(5):468-480(2003)
  • Kenmochi, N., et al. Genomics 77 (1-2), 65-70 (2001) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This MRPL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-264 amino acids from the C-terminal region of human MRPL2.
NCBI Gene ID #:
51069
NCBI Official Name:
39S ribosomal protein L2, mitochondrial
NCBI Official Symbol:
MRPL2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
33 kDa
Protein Accession #:
Q5T653
Protein GI Number:
118573672
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity
Swissprot #:
Q5T653
User NOte:
Optimal dilutions for each application to be determined by the researcher.