Further Information
Guanylyl cyclase-activating protein 1, GCAP 1, Guanylate cyclase activator 1A, GUCA1A, C6orf131, GCAP, GCAP1, GUCA1
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Guanylate cyclase-activating protein is a l Ca(2+)-binding protein that upregulates synthesis of cGMP in photoreceptors. The known mammalian GCAPs are more than 90% similar, consisting of 201 to 205 amino acids, and containing 3 identically conserved Ca(2+)-binding sites. The GUCA1A gene, also termed GCAP1, is transcribed into a single 1.7-kb mRNA species detectable only in the retina. In a 4-generation British family with typical clinical features of autosomal dominant cone dystrophy a tyr99-to-cys mutation) in the GUCA1A gene has been identified. Another family with a pro50-to-leu mutation in GUCA1A demonstrated phenotypic variability ranging from mild photophobia to rod-cone dystrophy. The mutant protein could activate guanylate cyclase 1 (GUCY2D) and displayed similar calcium sensitivity to wildtype protein. However, there was a marked increase in the susceptibility to protease degradation and a reduction in the thermal stability of the pro50-to-leu mutation, which may depress cellular concentration and thereby contribute to retinal cell mortality.
- Pennesi, M.E., et al., Proc. Natl. Acad. Sci. U.S.A. 100(11):6783-6788 (2003).
- Payne, A.M., et al., Hum. Mol. Genet. 7(2):273-277 (1998).
- Subbaraya, I., et al., J. Biol. Chem. 269(49):31080-31089 (1994).
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Bovine
This GCAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-191 amino acids from the C-terminal region of human GCAP1.
2978
Guanylyl cyclase-activating protein 1
GUCA1A
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
23 kDa
P43080
46577585
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Neuroscience,Signal Transduction
P43080
Optimal dilutions for each application to be determined by the researcher.