ProSci

GJA3 Antibody

Product Code:
 
PSI-58-175
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of GJA3 Antibody in Jurkat cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma;
❮❯

Western blot analysis of GJA3 Antibody in Jurkat cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma;

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PSI-58-175-400ul400ul£626.00
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Further Information

Additional Names:
Gap junction alpha-3 protein, Connexin-46, Cx46, GJA3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
GJA3, an integral membrane protein, belong to the connexin family, alpha-type (group II) subfamily. One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. A connexon is composed of a hexamer of connexins. This particular connexin is a component of lens fiber gap junctions, can form both junctional and nonjunctional ('hemi-') channels. Defects in GJA3 are the cause of zonular pulverulent cataract type 3 (CZP3), a form of autosomal dominant congenital cataract.
Background References:
  • Mackay, D., et al., Am. J. Hum. Genet. 64(5):1357-1364 (1999).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This GJA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-139 amino acids from the N-terminal region of human GJA3.
NCBI Gene ID #:
2700
NCBI Official Name:
Gap junction alpha-3 protein
NCBI Official Symbol:
GJA3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
47 kDa
Protein Accession #:
Q9Y6H8
Protein GI Number:
311033478
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
Q9Y6H8
User NOte:
Optimal dilutions for each application to be determined by the researcher.