ProSci

MID2 Antibody

Product Code:
 
PSI-57-170
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
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MID2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
MID2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-57-170-400ul400ul£626.00
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Further Information

Additional Names:
Probable E3 ubiquitin-protein ligase MID2, 632-, Midin-2, Midline defect 2, Midline-2, RING finger protein 60, Tripartite motif-containing protein 1, MID2, FXY2, RNF60, TRIM1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
Background References:
  • Lim, J., et al. Cell 125(4):801-814(2006)
  • Jehee, F.S., et al. Am. J. Med. Genet.139(3):221-226(2005)
  • Yap, M.W., et al. Proc. Natl. Acad. Sci. U.S.A. 101(29):10786-10791(2004)
  • Short, K.M., et al. BMC Cell Biol. 3,(2002) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This MID2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 690-717 amino acids from the C-terminal region of human MID2.
NCBI Gene ID #:
11043
NCBI Official Name:
Probable E3 ubiquitin-protein ligase MID2
NCBI Official Symbol:
MID2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
83 kDa
Protein Accession #:
Q9UJV3
Protein GI Number:
294862489
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
Q9UJV3
User NOte:
Optimal dilutions for each application to be determined by the researcher.