ProSci

ITGAX Antibody

Product Code:
 
PSI-62-571
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of ITGAX Antibody in MDA-MB231 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human hepatocarcinoma with ITGAX Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Immunofluorescence analysis of ITGAX Antibody with paraffin-embedded human hepatocarcinoma . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
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Western blot analysis of ITGAX Antibody in MDA-MB231 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma with ITGAX Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Immunofluorescence analysis of ITGAX Antibody with paraffin-embedded human hepatocarcinoma . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.

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PSI-62-571-400ul400ul£626.00
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Further Information

Additional Names:
Integrin alpha-X, CD11 antigen-like family member C, Leu M5, Leukocyte adhesion glycoprotein p150,95 alpha chain, Leukocyte adhesion receptor p150, CD11c, ITGAX, CD11C
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
ITGAX is the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles.
Background References:
  • Bulloch,K., et.al., J. Comp. Neurol. 508 (5), 687-710 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ITGAX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1119-1145 amino acids from the C-terminal region of human ITGAX.
NCBI Gene ID #:
3687
NCBI Official Name:
Integrin alpha-X
NCBI Official Symbol:
ITGAX
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
128 kDa
Protein Accession #:
P20702
Protein GI Number:
146345441
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology,Signal Transduction
Swissprot #:
P20702
User NOte:
Optimal dilutions for each application to be determined by the researcher.