ProSci

ACTR3 Antibody

Product Code:
 
PSI-61-583
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of ACTR3 Antibody in A2058 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ACTR3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of ACTR3 Antibody in A2058 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ACTR3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-61-583-400ul400ul£626.00
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Further Information

Additional Names:
Actin-related protein 3, Actin-like protein 3, ACTR3, ARP3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
ACTR3 is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion.
Background References:
  • Weisswange,I., et. al., Nature 458 (7234), 87-91 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse, Rat
Immunogen:
This ACTR3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-407 amino acids from the C-terminal region of human ACTR3.
NCBI Gene ID #:
10096
NCBI Official Name:
Actin-related protein 3
NCBI Official Symbol:
ACTR3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
47 kDa
Protein Accession #:
P61158
Protein GI Number:
47117647
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Immunology
Swissprot #:
P61158
User NOte:
Optimal dilutions for each application to be determined by the researcher.