ProSci

LY75 Antibody

Product Code:
 
PSI-63-736
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of LY75 Antibody in HL-60 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human spleen tissue reacted with LY75 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of LY75 Antibody in HL-60 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human spleen tissue reacted with LY75 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-63-736-400ul400ul£626.00
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Further Information

Additional Names:
Lymphocyte antigen 75, Ly-75, C-type lectin domain family 13 member B, DEC-205, gp200-MR6, CD205, LY75, CD205, CLEC13B
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
LY75 acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment. It causes reduced proliferation of B-lymphocytes.
Background References:
  • Kato,M., et.al., Immunogenetics 47 (6), 442-450 (1998)
  • McKay,P.F., et.al., Eur. J. Immunol. 28 (12), 4071-4083 (1998)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This LY75 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 646-675 amino acids from the Central region of human LY75.
NCBI Gene ID #:
100526664, 4065
NCBI Official Name:
Lymphocyte antigen 75
NCBI Official Symbol:
LY75
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
198 kDa
Protein Accession #:
O60449
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology
Swissprot #:
O60449
User NOte:
Optimal dilutions for each application to be determined by the researcher.