ProSci

VTN Antibody

Product Code:
 
PSI-62-933
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of VTN Antibody in NCI-H460(lane 1), HepG2(lane 2) cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human hepatocarcinoma with VTN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of VTN Antibody in NCI-H460(lane 1), HepG2(lane 2) cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma with VTN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-62-933-400ul400ul£626.00
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Further Information

Additional Names:
Vitronectin, VN, S-protein, Serum-spreading factor, V75, Vitronectin V65 subunit, Vitronectin V10 subunit, Somatomedin-B, VTN
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
VTN is a member of the pexin family. This protein is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. The protein is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.
Background References:
  • Jenne D.E., Stanley K.K.EMBO J. 4:3153-3157(1985)
  • Sigurdardottir O., Wiman B.Biochim. Acta 1208:104-110(1994)
  • Seiffert D., Loskutoff D.J.J. Biol. Chem. 266:2824-2830(1991)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This VTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-379 amino acids from the C-terminal region of human VTN.
NCBI Gene ID #:
7448
NCBI Official Name:
Vitronectin
NCBI Official Symbol:
VTN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
54 kDa
Protein Accession #:
P04004
Protein GI Number:
139653
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Immunology
Swissprot #:
P04004
User NOte:
Optimal dilutions for each application to be determined by the researcher.