ProSci

GSS Antibody

Product Code:
 
PSI-62-525
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 5
Immunohistochemical analysis of paraffin-embedded H. stomach section using GSS Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
2 / 5
Immunohistochemical analysis of paraffin-embedded H. stomach section using GSS Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
3 / 5
4 / 5
Western blot analysis in Hela,293T,MDA-MB-453 cell line and mouse liver tissue lysates (35ug/lane).
5 / 5
Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).
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Immunohistochemical analysis of paraffin-embedded H. stomach section using GSS Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. stomach section using GSS Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis in Hela,293T,MDA-MB-453 cell line and mouse liver tissue lysates (35ug/lane).
Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).

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PSI-62-525-400ul400ul£662.00
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Further Information

Additional Names:
Glutathione synthetase, GSH synthetase, GSH-S, Glutathione synthase, GSS
Application Note:
For IHC-P starting dilution is: 1:25

For IF starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. GSS functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione.
Background References:
  • Starr,J.M., et.al., Mech. Ageing Dev. 129 (12), 745-751 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Monkey, Bovine
Immunogen:
This GSS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 372-400 amino acids from the C-terminal region of human GSS.
NCBI Gene ID #:
2937
NCBI Official Name:
Glutathione synthetase
NCBI Official Symbol:
GSS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
52 kDa
Protein Accession #:
P48637
Protein GI Number:
1346191
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Obesity,Neuroscience,Signal Transduction
Swissprot #:
P48637
User NOte:
Optimal dilutions for each application to be determined by the researcher.