ProSci

Nucleostemin Antibody

Product Code:
 
PSI-57-452
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of GNL3 Antibody in HL60 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GNL3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of GNL3 Antibody in HL60 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GNL3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-57-452-400ul400ul£626.00
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Further Information

Additional Names:
Guanine nucleotide-binding protein-like 3, E2-induced gene 3 protein, Novel nucleolar protein 47, NNP47, Nucleolar GTP-binding protein 3, Nucleostemin, GNL3, E2IG3, NS
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
GNL3 is found in the nucleoli of embryonic stem cells, adult CNS stem cells, primitive cells in the bone marrow and cancer cells. It is not in the differentiated cells of most adult tissues. It has been suggested to play a role in controlling the cell-cycle progression in stem cells and cancer cells, and may be required to maintain the proliferative capacity of stem cells.
Background References:
  • Ma,H.,Mol. Biol. Cell 18 (7), 2630-2635 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Nucleostemin (GNL3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 458-487 amino acids from the C-terminal region of human Nucleostemin (GNL3).
NCBI Gene ID #:
26354
NCBI Official Name:
Guanine nucleotide-binding protein-like 3
NCBI Official Symbol:
GNL3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
62 kDa
Protein Accession #:
Q9BVP2
Protein GI Number:
229462872
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Cell Cycle,Neuroscience,Signal Transduction
Swissprot #:
Q9BVP2
User NOte:
Optimal dilutions for each application to be determined by the researcher.