ProSci

LRRC45 Antibody

Product Code:
 
PSI-55-810
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of lysates from human blood plasma tissue (from left to right),using LRRC45 Antibody .AP11368b was diluted at 1:1000 at each lane.
2 / 4
Immunofluorescent analysis of U251 cells, using LRRC45 Antibody . Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
3 / 4
4 / 4
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of lysates from human blood plasma tissue (from left to right),using LRRC45 Antibody .AP11368b was diluted at 1:1000 at each lane.
Immunofluorescent analysis of U251 cells, using LRRC45 Antibody . Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-55-810-400ul400ul£626.00
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Further Information

Additional Names:
Leucine-rich repeat-containing protein 45, LRRC45
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:25

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background References:
  • Olsen, J.V., et al. Cell 127(3):635-648(2006) Gerhard, D.S., et al. Genome Res. 14 (10B), 2121-2127 (2004) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This LRRC45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 615-643 amino acids from the C-terminal region of human LRRC45.
NCBI Gene ID #:
201255
NCBI Official Name:
Leucine-rich repeat-containing protein 45
NCBI Official Symbol:
LRRC45
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
76 kDa
Protein Accession #:
Q96CN5
Protein GI Number:
74760765
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q96CN5
User NOte:
Optimal dilutions for each application to be determined by the researcher.