ProSci

SEPT9 Antibody

Product Code:
 
PSI-62-182
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of lysates from A431, Hela cell line (from left to right), using SEPT9 Antibody at 1:1000 at each lane.
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Western blot analysis of lysates from human kidney and liver tissue lysate (from left to right), using SEPT9 Antibody at 1:1000 at each lane.
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Antibody is used in Western blot to detect SEPT9 in Jurkat cell lysate.
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SEPT9 Antibody (A555) immunohistochemistry analysis in formalin fixed and paraffin embedded kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SEPT9 Antibody (A555) for immunohistochemistry.
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Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of lysates from A431, Hela cell line (from left to right), using SEPT9 Antibody at 1:1000 at each lane.
Western blot analysis of lysates from human kidney and liver tissue lysate (from left to right), using SEPT9 Antibody at 1:1000 at each lane.
Antibody is used in Western blot to detect SEPT9 in Jurkat cell lysate.
SEPT9 Antibody (A555) immunohistochemistry analysis in formalin fixed and paraffin embedded kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SEPT9 Antibody (A555) for immunohistochemistry.
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-62-182-400ul400ul£662.00
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Further Information

Additional Names:
Septin-9, MLL septin-like fusion protein MSF-A, MLL septin-like fusion protein, Ovarian/Breast septin, Ov/Br septin, Septin D1, SEPT9, KIAA0991, MSF
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
The maf oncogene was identified by structural analysis of the AS42 avian transforming retrovirus genome. The Maf family is divided into two subclasses, large Mafs (vMaf, cMaf, MafB and Nrl) and small Mafs (MafF, MafK, and MafG). Both subclasses contain leucinezipper motifs, which allow homodimerization as well as heterodimerization with a variety of other bZip transcription factors. Large Mafs also contain an acidic transactivation domain absent in the small Maf proteins. Although they do not possess inherent transactivation activity, small Maf proteins can act as positive regulators of transcription by targeting transcriptionally active dimerization partners to specific DNA regulatory elements. Conversely, small Mafs can act also as negative regulators of transcription by recruiting transcriptional repressors or by forming homodimers that can replace active dimers. Human MafF was isolated in a yeast one-hybrid system from a human myometrium cDNA library. Human MAFF encodes a 164 amino acids proten. Like other small MAFF proteins, it contains an extended leucine zipper structure and lacks an N-terminal transactivating domain. The three small Maf proteins have been implicated in a number of physiological processes, including development, differentiation, haematopoiesis and stress response. Interestingly, these three proteins regulate the stress response via different mechanisms.
Background References:
  • Proc. Natl. Acad. Sci. U.S.A. 96:6428-6433(1999). Cancer Res. 60: 4729-4734, 2000. Oncogene 20: 5930-5939, 2001.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SEPT9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-85 amino acids from the C-terminal region of human SEPT9.
NCBI Gene ID #:
10801
NCBI Official Name:
Septin-9
NCBI Official Symbol:
Sep-09
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
65 kDa
Protein Accession #:
Q9UHD8
Protein GI Number:
93141311
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q9UHD8
User NOte:
Optimal dilutions for each application to be determined by the researcher.