ProSci

CUL5 Antibody

Product Code:
 
PSI-63-007
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of anti-CUL5 Antibody in Hela cell line lysates (35ug/lane)
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Flow cytometric analysis of NCI-H292 cells using CUL5 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of anti-CUL5 Antibody in Hela cell line lysates (35ug/lane)
Flow cytometric analysis of NCI-H292 cells using CUL5 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-63-007-400ul400ul£626.00
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Further Information

Additional Names:
Cullin-5, CUL-5, Vasopressin-activated calcium-mobilizing receptor 1, VACM-1, CUL5, VACM1
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:50~100
Background:
CUL5 is a core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAk2. It seems to be involved poteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein and may form a cell surface vasopressin receptor.
Background References:
  • Kamura T., Burian D., Yan Q.J. Biol. Chem. 276:29748-29753(2001)
  • Mehle A., Goncalves J., Santa-Marta M.Genes Dev. 18:2861-2866(2004)
  • Kamura T., Maenaka K., Kotoshiba S.Genes Dev. 18:3055-3065(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rb
Immunogen:
This CUL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 747-774 amino acids from the C-terminal region of human CUL5.
NCBI Gene ID #:
8065
NCBI Official Name:
Cullin-5
NCBI Official Symbol:
CUL5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
91 kDa
Protein Accession #:
Q93034
Protein GI Number:
14917099
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle
Swissprot #:
Q93034
User NOte:
Optimal dilutions for each application to be determined by the researcher.