ProSci

WDR32 Antibody

Product Code:
 
PSI-55-300
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western blot analysis in A549 cell line lysates (35ug/lane).
2 / 3
WDR32 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3
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Western blot analysis in A549 cell line lysates (35ug/lane).
WDR32 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-55-300-400ul400ul£626.00
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Further Information

Additional Names:
DDB1- and CUL4-associated factor 10, WD repeat-containing protein 32, DCAF10, WDR32
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
DCAF10 may function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.
Background References:
  • Jin, J., et al. Mol. Cell 23(5):709-721(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This WDR32 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-142 amino acids from the N-terminal region of human WDR32.
NCBI Gene ID #:
79269
NCBI Official Name:
DDB1- and CUL4-associated factor 10
NCBI Official Symbol:
DCAF10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
61 kDa
Protein Accession #:
Q5QP82
Protein GI Number:
74762212
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q5QP82
User NOte:
Optimal dilutions for each application to be determined by the researcher.