ProSci

WFDC1 Antibody

Product Code:
 
PSI-56-449
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the WFDC1 antibody detected the WFDC1 protein (arrow).
2 / 3
immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3
❮❯

Western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the WFDC1 antibody detected the WFDC1 protein (arrow).
immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-56-449-400ul400ul£626.00
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Further Information

Additional Names:
WAP four-disulfide core domain protein 1, Prostate stromal protein ps20, ps20 growth inhibitor, WFDC1, PS20
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
This gene encodes a member of the WAP-type four disulfide core domain family. The WAP-type four-disulfide core domain, or WAP signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. The encoded protein shares 81% amino acid identity with the rat ps20 protein, which was originally identified as a secreted growth inhibitor. This gene is mapped to chromosome 16q24, an area of frequent loss of heterozygosity in cancers, including prostate, breast and hepatocellular cancers and Wilms' tumor. Owing to its location and a possible growth inhibitory property of its gene product, this gene is suggested to be a tumor suppressor gene.
Background References:
  • Briggs, F.B., et al. Genes Immun. 11(3):199-208(2010)
  • Madar, S., et al. Carcinogenesis 30(1):20-27(2009)
  • Liu, S., et al. Clin. Exp. Metastasis 26(7):739-749(2009)
  • Alvarez, R., et al. J. Virol. 82(1):471-486(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This WFDC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 148-177 amino acids from the C-terminal region of human WFDC1.
NCBI Gene ID #:
58189
NCBI Official Name:
WAP four-disulfide core domain protein 1
NCBI Official Symbol:
WFDC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
24 kDa
Protein Accession #:
Q9HC57
Protein GI Number:
22096244
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q9HC57
User NOte:
Optimal dilutions for each application to be determined by the researcher.