ProSci

SELT Antibody

Product Code:
 
PSI-63-886
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of SELT Antibody in MCF-7 cell line lysates (35ug/lane)
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Flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of SELT Antibody in MCF-7 cell line lysates (35ug/lane)
Flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-63-886-400ul400ul£626.00
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Further Information

Additional Names:
Selenoprotein T, SelT, SELT
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
SELT encodes a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.
Background References:
  • Kryukov,G.V., et.al., Science 300 (5624), 1439-1443 (2003)
  • Kryukov,G.V., et.al., J. Biol. Chem. 274 (48), 33888-33897 (1999)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse, Rat
Immunogen:
This SELT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-75 amino acids from the N-terminal region of human SELT.
NCBI Gene ID #:
51714
NCBI Official Name:
Selenoprotein T
NCBI Official Symbol:
SELT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
22 kDa
Protein Accession #:
P62341
Protein GI Number:
190358765
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
P62341
User NOte:
Optimal dilutions for each application to be determined by the researcher.