ProSci

HECTD3 Antibody

Product Code:
 
PSI-59-211
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in A549 cell line lysates (35ug/lane).
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Western blot analysis in A549 cell line lysates (35ug/lane).

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PSI-59-211-400ul400ul£626.00
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Further Information

Additional Names:
E3 ubiquitin-protein ligase HECTD3, 632-, HECT domain-containing protein 3, HECTD3
Application Note:
For WB starting dilution is: 1:1000
Background:
E3 ubiquitin ligases accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of TRIOBP and its subsequent proteasomal degradation, thus faciliting cell cycle progression by regulating the turn-over of TRIOBP. Mediates also ubiquitination of STX8 (By similarity).
Background References:
  • Yu, J., et al. Biochem. Biophys. Res. Commun. 367(4):805-812(2008)
  • Gevaert, K., et al. Nat. Biotechnol. 21(5):566-569(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HECTD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 740-769 amino acids from the C-terminal region of human HECTD3.
NCBI Gene ID #:
79654
NCBI Official Name:
E3 ubiquitin-protein ligase HECTD3
NCBI Official Symbol:
HECTD3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
97 kDa
Protein Accession #:
Q5T447
Protein GI Number:
74744877
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q5T447
User NOte:
Optimal dilutions for each application to be determined by the researcher.