ProSci

RNF128 Antibody

Product Code:
 
PSI-60-487
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).
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Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).

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PSI-60-487-400ul400ul£626.00
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Further Information

Additional Names:
E3 ubiquitin-protein ligase RNF128, 632-, Gene related to anergy in lymphocytes protein, GRAIL, RING finger protein 128, RNF128
Application Note:
For WB starting dilution is: 1:1000
Background:
The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Background References:
  • Su, L.L., et al. J. Immunol. 183(1):438-444(2009)
  • Lin, J.T., et al. J. Immunol. 182(10):5919-5928(2009)
  • Lineberry, N., et al. J. Biol. Chem. 283(42):28497-28505(2008)
  • Egawa, S., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 295 (1), G163-G169 (2008) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This RNF128 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-143 amino acids from the Central region of human RNF128.
NCBI Gene ID #:
79589
NCBI Official Name:
E3 ubiquitin-protein ligase RNF128
NCBI Official Symbol:
RNF128
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
47 kDa
Protein Accession #:
Q8TEB7
Protein GI Number:
74751443
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q8TEB7
User NOte:
Optimal dilutions for each application to be determined by the researcher.