ProSci

PGK1 Antibody

Product Code:
 
PSI-62-700
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
Western blot analysis of lysates from A431, Hela, U-937, BA/F3 cell line (from left to right), using PGK1 Antibody (S320) at 1:1000 at each lane.
2 / 2
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
❮❯

Western blot analysis of lysates from A431, Hela, U-937, BA/F3 cell line (from left to right), using PGK1 Antibody (S320) at 1:1000 at each lane.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-62-700-400ul400ul£662.00
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Further Information

Additional Names:
Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2, PGK1, PGKA
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Also known as ATP:3-phosphoglycerate 1-phosphotransferase, this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. Phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and is proposed to participate in the angiogenic process as a disulfide reductase. Mutations in PGK1 may be associated with hemolytic anemia.
Background References:
  • Shetty, S., et al., Am. J. Respir. Cell Mol. Biol. 31(1):100-106 (2004).
  • Daly, E.B., et al., Biochim. Biophys. Acta 1691(1):17-22 (2004).
  • Daly, E.B., et al., Int. J. Biol. Markers 19(2):170-172 (2004).
  • Saito, Y., et al., Biochem. Biophys. Res. Commun. 314(2):396-402 (2004).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This PGK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-334 amino acids from the Central region of human PGK1.
NCBI Gene ID #:
5230
NCBI Official Name:
Phosphoglycerate kinase 1
NCBI Official Symbol:
PGK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
Protein Accession #:
P00558
Protein GI Number:
52788229
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
P00558
User NOte:
Optimal dilutions for each application to be determined by the researcher.