ProSci

GOT2 Antibody

Product Code:
 
PSI-63-724
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of lysates from Hela, HepG2, HL-60, Ramos, mouse brain cell line (from left to right), using GOT2 Antibody at 1:1000 at each lane.
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Flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of lysates from Hela, HepG2, HL-60, Ramos, mouse brain cell line (from left to right), using GOT2 Antibody at 1:1000 at each lane.
Flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-63-724-400ul400ul£626.00
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Further Information

Additional Names:
Aspartate aminotransferase, mitochondrial, mAspAT, Fatty acid-binding protein, FABP-1, Glutamate oxaloacetate transaminase 2, Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine--oxoglutarate transaminase 4, Kynurenine--oxoglutarate transaminase IV, Plasma membrane-associated fatty acid-binding protein, FABPpm, Transaminase A, GOT2
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
Background References:
  • Schiele,F., et.al., Clin. Chem. 35 (6), 926-930 (1989)
  • Watazu,Y., et.al., Clin. Chem. 36 (4), 687-689 (1990)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Monkey, Mouse, Pig, Rat
Immunogen:
This GOT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-61 amino acids from the N-terminal region of human GOT2.
NCBI Gene ID #:
2806
NCBI Official Name:
Aspartate aminotransferase, mitochondrial
NCBI Official Symbol:
GOT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
48 kDa
Protein Accession #:
P00505
Protein GI Number:
308153643
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
P00505
User NOte:
Optimal dilutions for each application to be determined by the researcher.