ProSci

EGLN3 Antibody

Product Code:
 
PSI-57-569
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in HL-60 cell line lysates (35ug/lane).
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EGLN3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in HL-60 cell line lysates (35ug/lane).
EGLN3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-57-569-400ul400ul£626.00
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Further Information

Additional Names:
Egl nine homolog 3, HPH-1, Hypoxia-inducible factor prolyl hydroxylase 3, HIF-PH3, HIF-prolyl hydroxylase 3, HPH-3, Prolyl hydroxylase domain-containing protein 3, PHD3, EGLN3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
EGLN3 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. Promotes cell death through a caspase-dependent mechanism (By similarity).
Background References:
  • Sato, M., et al. Exp. Cell Res. 316(17):2871-2882(2010)
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Xue, J., et al. Gastroenterology 138(2):606-615(2010)
  • Henze, A.T., et al. Cancer Res. 70(1):357-366(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This EGLN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 203-231 amino acids from the C-terminal region of human EGLN3.
NCBI Gene ID #:
112399
NCBI Official Name:
Egl nine homolog 3
NCBI Official Symbol:
EGLN3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
27 kDa
Protein Accession #:
Q9H6Z9
Protein GI Number:
32129515
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity
Swissprot #:
Q9H6Z9
User NOte:
Optimal dilutions for each application to be determined by the researcher.