ProSci

SQLE Antibody

Product Code:
 
PSI-56-547
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in A549 cell line lysates (35ug/lane).
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SQLE Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in A549 cell line lysates (35ug/lane).
SQLE Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-56-547-400ul400ul£626.00
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Further Information

Additional Names:
Squalene monooxygenase, Squalene epoxidase, SE, SQLE, ERG1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Squalene epoxidase catalyzes the first oxygenation step in sterol biosynthesis and is thought to be one of the rate-limiting enzymes in this pathway.
Background References:
  • Bailey, S.D., et al. Diabetes Care (2010) In press :
  • Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
  • Lu, Y., et al. J. Lipid Res. 49(12):2582-2589(2008)
  • Helms, M.W., et al. Br. J. Cancer 99(5):774-780(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SQLE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from the N-terminal region of human SQLE.
NCBI Gene ID #:
6713
NCBI Official Name:
Squalene monooxygenase
NCBI Official Symbol:
SQLE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
64 kDa
Protein Accession #:
Q14534
Protein GI Number:
296439362
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity
Swissprot #:
Q14534
User NOte:
Optimal dilutions for each application to be determined by the researcher.