ProSci

HIF1A Antibody

Product Code:
 
PSI-60-903
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in A549 cell line lysates (35ug/lane).
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Western blot analysis in A549 cell line lysates (35ug/lane).

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PSI-60-903-400ul400ul£626.00
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Further Information

Additional Names:
Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8, HIF1A, BHLHE78, MOP1, PASD8
Application Note:
For WB starting dilution is: 1:1000
Background:
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.
Background References:
  • Gonsalves, C., et al. J. Immunol. 185(10):6253-6264(2010)
  • Hindryckx, P., et al. J. Immunol. 185(10):6306-6316(2010)
  • Espinosa, I., et al. Am. J. Surg. Pathol. 34(11):1708-1714(2010)
  • Corzo, C.A., et al. J. Exp. Med. 207(11):2439-2453(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This HIF1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 735-762 amino acids from the C-terminal region of human HIF1A.
NCBI Gene ID #:
3091
NCBI Official Name:
Hypoxia-inducible factor 1-alpha
NCBI Official Symbol:
HIF1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
93 kDa
Protein Accession #:
Q16665
Protein GI Number:
2498017
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity
Swissprot #:
Q16665
User NOte:
Optimal dilutions for each application to be determined by the researcher.