ProSci

KARS Antibody

Product Code:
 
PSI-55-623
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western blot analysis in MCF-7 cell line lysates (35ug/lane).
2 / 3
Western blot analysis of KARS using rabbit polyclonal KARS Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KARS gene.
3 / 3
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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Western blot analysis of KARS using rabbit polyclonal KARS Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KARS gene.

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PSI-55-623-400ul400ul£626.00
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Further Information

Additional Names:
Lysine--tRNA ligase, Lysyl-tRNA synthetase, LysRS, KARS, KIAA0070
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Background References:
  • McLaughlin, H.M., et al. Am. J. Hum. Genet. 87(4):560-566(2010)
  • Kepp, O., et al. Cell Cycle 9(15):3072-3077(2010)
  • Segat, L., et al. Vaccine 28(10):2201-2206(2010)
  • Dastani, Z., et al. Eur. J. Hum. Genet. 18(3):342-347(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This KARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-98 amino acids from the N-terminal region of human KARS.
NCBI Gene ID #:
3735
NCBI Official Name:
Lysine--tRNA ligase
NCBI Official Symbol:
KARS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
68 kDa
Protein Accession #:
Q15046
Protein GI Number:
20178333
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Neuroscience,Signal Transduction
Swissprot #:
Q15046
User NOte:
Optimal dilutions for each application to be determined by the researcher.