Further Information
Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27, HSPB1, HSP27, HSP28
For WB starting dilution is: 1:1000
In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSBP1 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoters (e.g. phorbol esters) and calcium ionophores, and high levels are associated with carcinoma of the breast and with endometrial adenocarcinomas. Heat shock of HeLa cell cultures, or treatment with arsenite, phorbol ester, or tumor necrosis factor, causes a rapid phosphorylation of preexisting HSBP1, with Ser82 as the major site and Ser78 the minor site of phosphorylation. HSBP1 may exert phosphorylation-activated functions linked with growth signaling pathways in unstressed cells. A homeostatic function at this level could protect cells from adverse effects of signal transduction systems which may be activated inappropriately during stress.
- Wano, C., et al., Exp. Cell Res. 298(2):584-592 (2004).
- Evgrafov, O.V., et al., Nat. Genet. 36(6):602-606 (2004).
- Song, H., et al., Biochem. Biophys. Res. Commun. 314(1):143-150 (2004).
- Chauhan, D., et al., Blood 102(9):3379-3386 (2003).
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
This HSP27(HSPB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-90 amino acids from human HSP27(HSPB1).
3315
Heat shock protein beta-1
HSPB1
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
23 kDa
P04792
19855073
This antibody is purified through a protein A column, followed by peptide affinity purification.
Cancer,Cell Cycle,Signal Transduction
P04792
Optimal dilutions for each application to be determined by the researcher.