ProSci

SOD1 Antibody

Product Code:
 
PSI-63-616
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of SOD1 Antibody in Jurkat cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human colon carcinoma reacted with SOD1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Confocal immunofluorescent analysis of SOD1 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
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Western blot analysis of SOD1 Antibody in Jurkat cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with SOD1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Confocal immunofluorescent analysis of SOD1 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

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PSI-63-616-400ul400ul£626.00
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Further Information

Additional Names:
Superoxide dismutase [Cu-Zn], Superoxide dismutase 1, hSod1, SOD1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
SOD1 binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein.
Background References:
  • Crapo,J.D., et.al., Proc. Natl. Acad. Sci. U.S.A. 89 (21), 10405-10409 (1992)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-84 amino acids from the Central region of human SOD1.
NCBI Gene ID #:
6647
NCBI Official Name:
Superoxide dismutase [Cu-Zn]
NCBI Official Symbol:
SOD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
16 kDa
Protein Accession #:
P00441
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Obesity,Signal Transduction,
Swissprot #:
P00441
User NOte:
Optimal dilutions for each application to be determined by the researcher.