Further Information
Mitogen-activated protein kinase 9, MAP kinase 9, MAPK 9, JNK-55, Stress-activated protein kinase 1a, SAPK1a, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2, MAPK9, JNK2, PRKM9, SAPK1A
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
JNK2 responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. JNK2 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it. JNK2 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. It is inhibited by dual specificity phosphatases, such as DUSP1. The protein has been shown to bind to at least three scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2 and MAPK8IP3/JIP-3/JSAP1. These proteins also bind other components of the JNK signaling pathway
- Gupta, S., et al., EMBO J. 15(11):2760-2770 (1996).
- Sluss, H.K., et al., Mol. Cell. Biol. 14(12):8376-8384 (1994).
- Kallunki, T., et al., Genes Dev. 8(24):2996-3007 (1994).
- Fleming, Y., et al., Biochem. J. 352 Pt 1, 145-154 (2000).
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
This JNK2 (MAPK9) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 349-379 amino acids from the C-terminal region of human JNK2 (MAPK9).
5601
Mitogen-activated protein kinase 9
MAPK9
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
48 kDa
P45984
85700366
This antibody is purified through a protein A column, followed by peptide affinity purification.
Cancer,Cell Cycle,Immunology,Obesity,Neuroscience,Signal Transduction
P45984
Optimal dilutions for each application to be determined by the researcher.