ProSci

KIT Antibody

Product Code:
 
PSI-60-071
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the KIT antibody detected the KIT protein (arrow).
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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the KIT antibody detected the KIT protein (arrow).

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PSI-60-071-400ul400ul£626.00
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Further Information

Additional Names:
Mast/stem cell growth factor receptor Kit, SCFR, Piebald trait protein, PBT, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, CD117, KIT, SCFR
Application Note:
For WB starting dilution is: 1:1000
Background:
This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K).
Background References:
  • Molderings, G.J., et al. Immunogenetics 62 (11-12), 721-727 (2010) :
  • Cheng, M., et al. Circ. Res. 107(9):1083-1093(2010)
  • Chi, P., et al. Nature 467(7317):849-853(2010)
  • Rossi, S., et al. Am. J. Surg. Pathol. 34(10):1480-1491(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This KIT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 340-369 amino acids from the Central region of human KIT.
NCBI Gene ID #:
3815
NCBI Official Name:
Mast/stem cell growth factor receptor Kit
NCBI Official Symbol:
KIT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
110 kDa
Protein Accession #:
P10721
Protein GI Number:
125472
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Immunology,Neuroscience,Signal Transduction
Swissprot #:
P10721
User NOte:
Optimal dilutions for each application to be determined by the researcher.