ProSci

PCNA Antibody

Product Code:
 
PSI-61-537
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of PCNA Antibody in Jurkat(lane 1), Hela(lane 2), 293(lane 3) cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PCNA antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of PCNA Antibody in Jurkat(lane 1), Hela(lane 2), 293(lane 3) cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PCNA antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-61-537-400ul400ul£626.00
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Further Information

Additional Names:
Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.
Background References:
  • Wang,Y., J. Cell. Biochem. 106 (3), 409-413 (2009)
  • Maga,G., Proc. Natl. Acad. Sci. U.S.A. 105 (52), 20689-20694 (2008)
  • Acharya,N., Proc. Natl. Acad. Sci. U.S.A. 105 (46), 17724-17729 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Hamster, Monkey, Mouse, Rat
Immunogen:
This PCNA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the Central region of human PCNA.
NCBI Gene ID #:
5111
NCBI Official Name:
Proliferating cell nuclear antigen
NCBI Official Symbol:
PCNA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
29 kDa
Protein Accession #:
P12004
Protein GI Number:
129694
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle
Swissprot #:
P12004
User NOte:
Optimal dilutions for each application to be determined by the researcher.