ProSci

PLG Antibody

Product Code:
 
PSI-62-815
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of PLG antibody in K562 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with PLG Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of PLG antibody in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with PLG Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-62-815-400ul400ul£626.00
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Further Information

Additional Names:
Plasminogen, Plasmin heavy chain A, Activation peptide, Angiostatin, Plasmin heavy chain A, short form, Plasmin light chain B, PLG
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
PLG is a circulating zymogen that is converted to the active enzyme plasmin by cleavage of the peptide bond between arg560 and val561, which is mediated by urokinase and tissue plasminogen activator. The main function of this protein is to dissolve fibrin clots. The protein, like trypsin, belongs to the family of serine proteinases.
Background References:
  • Hofmann,S.C., Voith,U. J. Invest. Dermatol. 129 (7), 1730-1739 (2009)
  • Passero,C.J., Mueller,G.M. J. Biol. Chem. 283 (52), 36586-36591 (2008)
  • Ohyama,S., Harada,T. Eur. J. Biochem. 271 (4), 809-820 (2004)
  • Lee,H., Kim,H.K. Arch. Biochem. Biophys. 375 (2), 359-363 (2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PLG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 636-665 amino acids from the C-terminal region of human PLG.
NCBI Gene ID #:
5340
NCBI Official Name:
Plasminogen
NCBI Official Symbol:
PLG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
91 kDa
Protein Accession #:
P00747
Protein GI Number:
130316
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle
Swissprot #:
P00747
User NOte:
Optimal dilutions for each application to be determined by the researcher.