ProSci

DLG7 Antibody

Product Code:
 
PSI-61-521
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of DLG7 Antibody in 293 cell line lysates (35ug/lane)
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DLG7 Antibody IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of DLG7 Antibody in 293 cell line lysates (35ug/lane)
DLG7 Antibody IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-61-521-400ul400ul£626.00
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Further Information

Additional Names:
Disks large-associated protein 5, DAP-5, Discs large homolog 7, Disks large-associated protein DLG7, Hepatoma up-regulated protein, HURP, DLGAP5, DLG7, KIAA0008
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
DLG7 is potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. DLG7 is mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. DLG7 is key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.
Background References:
  • de Reynies, A., et al. J. Clin. Oncol. 27(7):1108-1115(2009) Wong, J., et al. Mol. Biol. Cell 19(5):2083-2091(2008) Gudmundsson, K.O., et al. Stem Cells 25(6):1498-1506(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This DLG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-258 amino acids from the Central region of human DLG7.
NCBI Gene ID #:
9787
NCBI Official Name:
Disks large-associated protein 5
NCBI Official Symbol:
DLGAP5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
95 kDa
Protein Accession #:
Q15398
Protein GI Number:
82592583
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle
Swissprot #:
Q15398
User NOte:
Optimal dilutions for each application to be determined by the researcher.