ProSci

Beta-Glucuronidase Recombinant Protein

Product Code:
 
PSI-92-300
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Regulatory Status:
 
RUO
Target Species:
 
Escherichia coli
Storage:
 
Store at -20˚C, stable for 6 months after receipt.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
 

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PSI-92-300-0.05mg0.05mg£673.00
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Further Information

Additional Names:
Beta-glucuronidase, uidA, GUS, Beta-GUS, GUSB
Application Note:
This recombinant protein can be used for biological assays. For research use only.
Background:
Beta-D-glucuronidase from E. coli is a ?highly specific enzyme in hydrolyzing glucuronides. Beta-D-glucuronidase (GLUase) activity can be measured as the rate of production of fluorescent methylumbelliferone (MU), resulting from the hydrolysis of the substrate 4-methylumbelliferyl- beta -d-glucuronide (MUGLU), which is an effective and rapid method for detection and verification of E. coli in food, water, and environmental samples. High purity recombinant beta-Glucuronidase is used in research, biochemical enzyme assays and in vitro diagnostic analysis, detecting a wide range of drugs such as opioids, benzodiazepines, steroids, cannabinoids, and others.
Buffer:
Supplied as a 0.2 um filtered solution of PBS,pH7.4. It is not recommended to reconstitute to a concentration less than 100 ug/ml.
Fusion Tag:
N-6 His tag
NCBI Gene ID #:
946149
NCBI Official Name:
beta-D-glucuronidase
NCBI Official Symbol:
uidA
NCBI Organism:
Escherichia coli str. K-12 substr. MG1655
Physical State:
Liquid
Predicted Molecular Weight:
69.9 kD
Protein Accession #:
P05804
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
P05804

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