RNA pyrophosphohydrolase Recombinant Protein

RNA pyrophosphohydrolase, (Di)nucleoside polyphosphate hydrolase, Ap5A pyrophosphatase, rppH

This recombinant protein can be used for biological assays. For research use only.

Messenger RNA (mRNA) degradation plays a key role in the control of gene expression in all organisms by limiting the number of times that each mRNA molecule can be used as a template for protein synthesis. RNA pyrophosphohydrolase, also called RppH, is a master regulator of 5'-dependent mRNA decay. It accelerates the degradation of transcripts by removing pyrophosphate from the 5'-end of triphosphorylated RNA, leading to a more labile monophosphorylated state that can stimulate subsequent ribonuclease cleavage. RppH preferentially hydrolyzes diadenosine penta-phosphate with ATP as one of the reaction products, and can be able to hydrolyze diadenosine hexa- and tetra-phosphate. However, this protein has no activity on diadenosine tri-phosphate, ADP-ribose, NADH and UDP-glucose. In the meningitis causing strain E. coli K1, it has been shown to play a role in HBMEC (human brain microvascular endothelial cells) invasion in vitro.

Supplied as a 0.2 um filtered solution of 50mM Tris, 500mM NaCl, 10% glycerol, pH8.0. It is not recommended to reconstitute to a concentration less than 100 ug/ml.

Tag Free

947300

RNA pyrophosphohydrolase

rppH

Escherichia coli str. K-12 substr. MG1655

Liquid

20.8 kD

P0A776

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.

Thr32-Gly402

E. coli

P0A776