ProSci

MED12 Antibody

Product Code:
 
PSI-43-315
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
 

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PSI-43-315-0.1mg0.1mg£505.00
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Further Information

Additional Names:
MED12, mediator complex subunit 12, ARC240, CAGH45, FGS1, HOPA, KIAA0192, OKS, OPA1, TNRC11, TRAP230, CAG repeat protein 45, OPA-containing protein, OTTHUMP00000023490, OTTHUMP00000023493, activator-recruited cofactor 240 kDa component, human opposite paired, mediator of RNA polymerase II transcription subunit 12, mediator of RNA polymerase II transcription, subunit 12 homolog, thyroid hormone receptor-associated protein complex 230 kDa component, thyroid hormone receptor-associated protein, 230 kDa subunit, trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit), trinucleotide repeat-containing gene 11 protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 70kDa band in Human Brain (Olfactory Bulb) and Rat Brain lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 243kDa according to NP_005111.2. The 70kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Xu X, Zhou H, Boyer TG.Mediator is a transducer of amyloid-precursor-protein-dependent nuclear signalling.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-NPTLVDPTRHLQQR
NCBI Gene ID #:
9968
NCBI Official Name:
mediator complex subunit 12
NCBI Official Symbol:
MED12
NCBI Organism:
Homo Sapiens
Physical State:
Liquid
Protein Accession #:
NP_005111.2
Protein GI Number:
110347429
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology
Swissprot #:
Q93074