Further Information
CENPE, centromere protein E, 312kDa, CENP-E, KIF10, PPP1R61, Centromere autoantigen E (312kD), centromere-associated protein E, kinesin family member 10, kinesin-related protein CENPE, protein phosphatase 1, regulatory subunit 61
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 316kDa according to NP_001804.2. Therefore we cannot recommend an optimal concentration and the antibody is an aspiring product. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
- Sardar HS, Gilbert SP.Microtubule capture by mitotic kinesin centromere protein E (CENP-E).
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Human
The immunogen for this antibody is: KREAHKQVTCEN
1062
centromere protein E, 312kDa
CENPE
Homo sapiens
Liquid
NP_001804.2, NP_001273663.1
71061468
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer,Cancer
This antibody is expected to recognize both reported isoforms (NP_001804.2; NP_001273663.1).
Q02224