ProSci

MAP3K6 Antibody

Product Code:
 
PSI-43-133
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Porcine (pig)
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
43-133 (0.3ug/ml) staining of Rat Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-133 (0.3ug/ml) staining of Rat Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-43-133-0.1mg0.1mg£626.00
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Further Information

Additional Names:
MAP3K6, mitogen-activated protein kinase kinase kinase 6, ASK2, MAPKKK6, MGC125653, MGC20114, OTTHUMP00000005063, apoptosis signal-regulating kinase 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 140kDa band observed in Rat Heart lysates (calculated MW of 143kDa according to Human NP_004663.3 and Rat NP_001101379.1). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Eto N, Miyagishi M, Inagi R, Fujita T, Nangaku M.Mitogen-activated protein 3 kinase 6 mediates angiogenic and tumorigenic effects via vascular endothelial growth factor expression.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-RAILAQRAGSTP
NCBI Gene ID #:
9064
NCBI Official Name:
mitogen-activated protein kinase kinase kinase 6?
NCBI Official Symbol:
MAP3K6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 140kDa
Protein Accession #:
NP_004663.3
Protein GI Number:
45827809
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
O95382