Further Information
Cstf2, cleavage stimulation factor, 3' pre-RNA subunit 2, 64kDa, C630034J23Rik, Cstf64, CF-1 64 kDa subunit, CSTF 64 kDa subunit, alphaCstF-64 variant 4, betaCstF-64 variant 1, betaCstF-64 variant 3, cleavage stimulation factor 64 kDa subunit, cleavage stimulation factor subunit 2, cleavage stimulation factor, 3' pre-RNA subunit 2, 64 kDa, cstF-64
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Immunoprecipitation: In transfected HeLa transiently expressing Mouse ?Cstf-64 ((DYKDDDDK tagged), a band of approx. 80kDa was observed using anti-DYKDDDDK antibody in Western blot after immunoprecipitation with EB11838. No bands were observed in mock-transfected HeLa. Yields were similar between RIPA buffer and Triton X-100 buffer conditions. Recommended amount, 2ug per lysate.
Immunoprecipitation: An approx 75kDa band is observed when endogenously expressed ?Cstf-64 is pulled down from Mouse Brain lysates using EB11838, while Cstf-64 which is endogenously expresed in Mouse Liver, is not pulled down. Western blot was probed with a mouse monoclonal antibody recognizing both ?Cstf-64 anf Cstf-64 Recommended amount 2ug EB11838/500ug lysate protein.
- Shankarling GS, Coates PW, Dass B, Macdonald CC.A family of splice variants of CstF-64 expressed in vertebrate nervous systems.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
The immunogen for this antibody is: C-PASIERVQGQRT
108062
cleavage stimulation factor, 3' pre-RNA subunit 2
Cstf2
Mus musculus
Liquid
ACF05702.1, ACF05700.1
192764316
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Signal Transduction,Stem Cell
This antibody recognizes mouse variants 1 and 3 of the reported beta isoform (ACF05702.1; ACF05700.1).
Q8BIQ5