ProSci

p47phox Antibody

Product Code:
 
PSI-43-227
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
43-227 (0.03ug/ml) staining of Mouse Thymus lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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43-227 (0.03ug/ml) staining of Mouse Thymus lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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PSI-43-227-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
Ncf1, neutrophil cytosolic factor 1, NOXO2, Ncf-1, p47, p47phox, 47 kDa neutrophil oxidase factor, NADPH oxidase subunit (47 kD), NADPH oxidase subunit (47kDa), NCF-47K, OTTMUSP00000022112, neutrophil NADPH oxidase factor 1, neutrophil cytosol factor 1, p47-phox
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 40kDa band observed in Mouse Thymus lysates (calculated MW of 44.7kDa according to NP_035006.3). Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Lung, Thymus and Spleen and U937 for this particular batch.
Background References:
  • Roos D, Kuhns DB, Maddalena A, Bustamante J, Kannengiesser C, de Boer M, van Leeuwen K, Köker MY, Wolach B, Roesler J, Malech HL, Holland SM, Gallin JI, Stasia MJ, Hematologically important mutations: the autosomal recessive forms of chronic granulomatous disease (second update).
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-DEAEDPDPNYAGEP
NCBI Gene ID #:
653361
NCBI Official Name:
neutrophil cytosolic factor 1
NCBI Official Symbol:
Ncf1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 45kDa
Protein Accession #:
NP_035006.3
Protein GI Number:
115298672
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis,Homeostasis
Swissprot #:
P14598