ProSci

PIM2 Antibody

Product Code:
 
PSI-43-337
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PIM2 with C-terminal MYC tag probed with 43-337 (0.1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 43-337 (0.1mg/ml) in Lane B. Detec
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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PIM2 with C-terminal MYC tag probed with 43-337 (0.1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 43-337 (0.1mg/ml) in Lane B. Detec

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PSI-43-337-0.1mg0.1mg£626.00
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Further Information

Additional Names:
PIM2, pim-2 oncogene, pim-2h, proto-oncogene Pim-2 (serine threonine kinase), serine/threonine protein kinase pim-2, serine/threonine-protein kinase pim-2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:In transfected HEK293 transiently expressing Human PIM2 (myc and DYKDDDDK tagged), a band of approx. 38kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration: 0.5-1ug/ml. Primary incubations were for 1 hour.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 5ug/ml.
Background References:
  • Morishita D, Katayama R, Sekimizu K, Tsuruo T, Fujita N, Pim kinases promote cell cycle progression by phosphorylating and down-regulating p27Kip1 at the transcriptional and posttranscriptional levels.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-QTPAEDVPLNPSK
NCBI Gene ID #:
11040
NCBI Official Name:
Pim-2 proto-oncogene, serine/threonine kinase
NCBI Official Symbol:
PIM2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006866.2
Protein GI Number:
42821112
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience
Swissprot #:
Q9P1W9