ProSci

TNNT1 Antibody

Product Code:
 
PSI-43-214
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
43-214 (0.03ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-214 (0.03ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-43-214-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
TNNT1, troponin T type 1 (skeletal, slow), ANM, FLJ98147, MGC104241, STNT, TNT, TNTS, slow skeletal muscle troponin T, troponin T, slow skeletal muscle, troponin-T1, skeletal, slow
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 37+35kDa bands observed in Human Skeletal Muscle lysates A single 37kDa band was observed in some lysates.(calculated MW of 32.9kDa according to NP_003274.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03-0.1ug/ml.
Background References:
  • Midde K, Dumka V, Pinto JR, Muthu P, Marandos P, Gryczynski I, Gryczynski Z, Potter JD, Borejdo J.Myosin cross-bridges do not form precise rigor bonds in hypertrophic heart muscle carrying troponin T mutations.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-EKAQELSDWIHQLE
NCBI Gene ID #:
7138
NCBI Official Name:
troponin T type 1 (skeletal, slow)
NCBI Official Symbol:
TNNT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 37 + 35kDa
Protein Accession #:
NP_003274.3, NP_001119604.1, NP_001119605.1
Protein GI Number:
187173288
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Obesity
SPECIFICITY:
This antibody is expected to recognize all reported isoforms (NP_003274.3; NP_001119604.1; NP_001119605.1).
Swissprot #:
P13805