Further Information
AIFM1, apoptosis-inducing factor, mitochondrion-associated, 1, AIF, CMTX4, COWCK, COXPD6, PDCD8, apoptosis-inducing factor 1, mitochondrial, programmed cell death 8 (apoptosis-inducing factor), striatal apoptosis-inducing factor
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in lysates of cell line Jurkat (calculated MW of 66.9kDa according to NP_004199.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence:?Strong expression of the protein seen in the Mitochondria of HeLa and NIH3T3 cells. Recommended concentration: 5-10ug/ml.
- Lewis EM, Wilkinson AS, Jackson JS, Mehra R, Varambally S, Chinnaiyan AM, Wilkinson JC.The enzymatic activity of apoptosis-inducing factor supports energy metabolism benefiting the growth and invasiveness of advanced prostate cancer cells.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Cow
The immunogen for this antibody is: C-DDPNVTKTLRFKQ
9131
apoptosis-inducing factor, mitochondrion-associated, 1
AIFM1
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 70kDa
NP_004199.1, NP_665811.1, NP_001124319.1
22202631
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Homeostasis,Homeostasis
This antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1) and isoform 5 (NP_001124319).
O95831-2