ProSci

CP110 Antibody

Product Code:
 
PSI-43-253
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
43-253 (1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-253 (1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-43-253-0.1mg0.1mg£626.00
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Further Information

Additional Names:
CP110, -, Cep110, DKFZp781G1416, KIAA0419, OTTHUMP00000162162, centrosomal protein CP110, centrosomal protein of 110 kDa
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 110kDa band observed in Human Testis lysates (calculated MW of 113kDa according to NP_001185951.1). Recommended concentration: 0.3-1ug/ml. Additional bands of unknown identity were also consistently observed at 65kDa and 37kDa. These bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/specific breakdown fragments been reported?
Background References:
  • D'Angiolella V, Donato V, Vijayakumar S, Saraf A, Florens L, Washburn MP, Dynlacht B, Pagano M.SCF(Cyclin F) controls centrosome homeostasis and mitotic fidelity through CP110 degradation.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: CEKSLARIQEAS
NCBI Gene ID #:
9738
NCBI Official Name:
centriolar coiled coil protein 110kDa
NCBI Official Symbol:
CP110
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 110kDa
Protein Accession #:
NP_001185951.1, NP_055526.3
Protein GI Number:
312222737
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis,Homeostasis
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_001185951.1; NP_055526.3).
Swissprot #:
O43303