ProSci

YAP1 Antibody

Product Code:
 
PSI-43-135
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Canine (dog)
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
43-135 (0.5ug/ml) staining of MDCK lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-135 (0.5ug/ml) staining of MDCK lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-43-135-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
Yes-associated protein 1, 65kDa, YAP, YAP2, YAP65, Yes-associated protein 1, 65 kD, Yes-associated protein 1, 65 kDa, yes-associated protein 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 70kDa band observed in lysates of cell line MDCK (calculated MW of 50.3kDa according to Dog XP_536601.2). Data obtained from an anonymous customer. Recommended concentration: 0.5-2ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Background References:
  • Zender L, Spector MS, Xue W, Flemming P, Cordon-Cardo C, Silke J, Fan ST, Luk JM, Wigler M, Hannon GJ, Mu D, Lucito R, Powers S, Lowe SW.Identification and validation of oncogenes in liver cancer using an integrative oncogenomic approach.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat,
Immunogen:
The immunogen for this antibody is: C-TYHSRDESTDS
NCBI Gene ID #:
10413
NCBI Official Name:
Yes-associated protein 1
NCBI Official Symbol:
YAP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 70kDa
Protein Accession #:
NP_006097.1
Protein GI Number:
194306653
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Transcription,Cancer Transcription
Swissprot #:
P46937