ProSci

HOOK3 Antibody

Product Code:
 
PSI-8425
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
HOOK3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
1 / 3
Western blot analysis of HOOK3 in mouse brain tissue lysate with HOOK3 antibody at 1 μg/ml.
2 / 3
Immunohistochemistry of HOOK3 in mouse brain tissue with HOOK3 antibody at 10 μg/ml.
3 / 3
Immunofluorescence of HOOK3 in mouse brain tissue with HOOK3 antibody at 20 μg/ml.<br><br>Red: HOOK3 Antibody (8425) <br> Blue: DAPI staining
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Western blot analysis of HOOK3 in mouse brain tissue lysate with HOOK3 antibody at 1 μg/ml.
Immunohistochemistry of HOOK3 in mouse brain tissue with HOOK3 antibody at 10 μg/ml.
Immunofluorescence of HOOK3 in mouse brain tissue with HOOK3 antibody at 20 μg/ml.<br><br>Red: HOOK3 Antibody (8425) <br> Blue: DAPI staining

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CodeSizePrice
PSI-8425-0.02mg0.02mg£150.00
Quantity:
PSI-8425-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
hook microtubule-tethering protein 3, HK3, h-hook3, hHK3, FLJ31058, Protein Hook homolog 3
Application Note:
HOOK3 antibody can be used for the detection of HOOK3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry at 10 μg/ml.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
The HOOK proteins (HOOK1, HOOK2 and HOOK3) comprise a family of cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules mediating the spatial organization of diverse membrane-trafficking systems; their more divergent C-terminal domains mediate binding to organelles (1,2). HOOK3 exists as a homodimer and participates in defining the architecture and localization of the Golgi complex through its central coiled-coil domain (3). HOOK3 may regulate clearance of endocytosed receptors such as MSR1 (3,4).
Background References:
  • Walenta JH, Didier AJ, Liu X, et al. The Golgi-associated hook3 protein is a member of a novel family of microtubule-binding proteins. J. Cell Biol. 2001; 152:923-34.
  • Sano H, Ishino M, Kr?mer H, et al. The microtubule-binding protein Hook3 interacts with a cytoplasmic domain of scavenger receptor A. J. Biol. Chem. 2007; 282:7973-81.
  • Herrmann L, Wiegmann C, Arsalan-Werner A, et al. Hook proteins: association with Alzheimer pathology and regulatory role of hook3 in amyloid beta generation. PLoS One 2015; 10:e0119423.
  • Ciampi R, Giordano TJ, Wikenheiser-Brokamp K, et al. HOOK3-RET: a novel type of RET/PTC rearrangement in papillary thyroid carcinoma. Endocr. Relat. Cancer 2007; 14:445-52.
Buffer:
HOOK3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
HOOK3 antibody was raised against a 19 amino acid peptide from near the amino terminus of human HOOK3.

The immunogen is located within amino acids 210 - 260 of HOOK3.
NCBI Gene ID #:
84376
NCBI Official Name:
Hook microtubule-tethering protein 3
NCBI Official Symbol:
HOOK3
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 79 kDa

Observed: 77 kDa
Protein Accession #:
NP_115786
Protein GI Number:
14165274
Purification:
HOOK3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
HOOK3 antibody is human, mouse and rat reactive. HOOK3 antibody is predicted to not cross-react with HOOK1 and HOOK2.
Swissprot #:
Q86VS8
User NOte:
Optimal dilutions for each application to be determined by the researcher.