CD8a Antibody [53-6.7] (Violet-450)
Antibody Isotype:
Rat IgG2a, kappa
Antibody Clonality:
Monoclonal
Application:
Flow Cytometry
Storage:
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
Ly-2, Ly-B, Ly-35, Lyt-2, BB154331, Cd8a
The 53-6.7 monoclonal antibody specifically reacts with Ly-2, the 38 kDa alpha chain, and with Lyt-2, the 34 kDa alpha? chain, of the mouse CD8 antigen. The alpha? chain is the truncated form of alpha chain, encoded by the same CD8a gene. In CD8a, the alpha and alpha? chains form heterodimers with CD8b (the beta chains) or homodimers (alpha-alpha), which occur as receptors on the surface of the majority of thymocytes. A subpopulation of mature T lymphocytes expresses the CD8 alpha beta (alpha beta TCR T cells), and a subpopulation of intestinal intraepithelial lymphocytes and dendritic cells express CD8a without CD8b.CD8 interacts with the mouse major histocompatibility complex class I (MHC class I) molecules on antigen-presenting cells or epithelial cells. Its function seems to be to attenuate the CD8-mediated signal for the stimulation of intrathymic T-cell maturation.The 53-6.7 antibody is useful for depleting CD8+ peripheral T lymphocytes. It cross reacts with the alpha- and alpha?-like polypeptides on some thymic and peripheral lymphocytes.BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.
- Ledbetter, J. A., Herzenberg, L. A. (1979). Xenogeneic Monoclonal Antibodies to Mouse Lymphoid Differentiation Antigens*.Immunological reviews,47(1), 63-90.
- Ledbetter, J. A., Rouse, R. V., Micklem, H. S., Herzenberg, L. A. (1980). T cell subsets defined by expression of Lyt-1, 2, 3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views.The Journal of experimental medicine,152(2), 280-295.
- Hathcock, K. (1991). T cell enrichment by cytotoxic elimination of B cells and accessory cells.Current protocols in immunology, 3-3.
Phosphate-buffered aqueous solution, ?0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
batch dependent
Violet-450
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
12525
CD8 antigen, alpha chain
Cd8a
Mus musculus
liquid
The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Immunology
The 53-6.7 monoclonal antibody specifically reacts with Ly-2, the 38 kDa alpha chain, and with Lyt-2, the 34 kDa alpha? chain, of the mouse CD8 antigen.
P01731
Optimal dilutions for each application to be determined by the researcher.