Further Information
Praja ring finger 1, E3 ubiquitin-protein ligase Praja-1, PRAJA1, RING finger protein 70, RNF70
PJA1 antibody can be used for detection of PJA1 by Western blot at 0.25 ? 0.5 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Ubiquitinization is an important cellular degradation process requiring sequential reactions that are mediated by three enzymes: E1, E2 and E3. PJA1, also known as Praja1 and RING finger protein 70, is a 643 amino acid E2-dependent E3-ubiquitin ligase that is abundantly expressed in the brain (1,2). Through interaction and activation with the E2-ubiquitin ligase UBC4, PJA1 mediates substrate-specific ubiquitination via its RING finger domain and facilitates ubiquitination (3). Overexpression of PJA1 in gastrointestinal cancers suggests that it may be responsible for the degradation of some anti-oncogenic proteins (4,5).
- Yu P, Chen Y, Tagle DA, et al. PJA1, encoding a RING-H2 finger ubiquitin ligase, is a novel human X chromosome gene abundantly expressed in brain. Genomics 2002; 79:869-74.
- Zoabi M, Sadeh R, de Bie P, et al. PRAJA1 is a ubiquitin ligase for the polycomb repressive complex 2 proteins. Biochem. Biophys. Res. Commun. 2011; 408:393-8.
- Doyle JM, Gao J, Wang J, et al. MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases. Mol. Cell 2010; 39:963-74.
- Saha T, Vardhini D, Tang Y, et al. RING finger-dependent ubiquitination by PRAJA is dependent on TGF-beta and potentially defines the functional status of the tumor suppressor ELF. Oncogene 2006; 25:693-705.
PJA1 antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
PJA1 antibody was raised against a 19 amino acid peptide near the amino terminus of human PJA1.
The immunogen is located within amino acids 50 - 100 of PJA1.
64219
praja ring finger 1, E3 ubiquitin protein ligase
PJA1
homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 71 kDa
Observed: 71 kDa
NP_660095
41281725
PJA1 antibody is affinity chromatography purified via peptide column.
Cancer,Homeostasis
PJA1 antibody is human, mouse and rat reactive. At least two isoforms of PJA1 are known to exist; this antibody will detect both isoforms.
Q8NG27
Optimal dilutions for each application to be determined by the researcher.