ProSci

HES5 Antibody

Product Code:
 
PSI-8055
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
HES5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
1 / 5
Western blot analysis of HES5 in Raji cell lysate with HES5 antibody at 1 μg/ml (A) the absence and (B) the presence of blocking peptide.
2 / 5
Immunohistochemistry of HES5 in human spleen tissue with HES5 antibody at 5 μg/ml.
3 / 5
Immunofluorescence of HES5 in human spleen tissue with HES5 antibody at 20 μg/ml.
4 / 5
Immunohistochemistry of HES5 in mouse brain tissue with HES5 Antibody at 5 μg/mL.
5 / 5
Immunofluorescence of HES5 in mouse brain tissue with HES5 Antibody at 20 μg/mL.<br><br>Green: HES5 Antibody (8055) <br> Blue: DAPI staining
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Western blot analysis of HES5 in Raji cell lysate with HES5 antibody at 1 μg/ml (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of HES5 in human spleen tissue with HES5 antibody at 5 μg/ml.
Immunofluorescence of HES5 in human spleen tissue with HES5 antibody at 20 μg/ml.
Immunohistochemistry of HES5 in mouse brain tissue with HES5 Antibody at 5 μg/mL.
Immunofluorescence of HES5 in mouse brain tissue with HES5 Antibody at 20 μg/mL.<br><br>Green: HES5 Antibody (8055) <br> Blue: DAPI staining

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CodeSizePrice
PSI-8055-0.02mg0.02mg£150.00
Quantity:
PSI-8055-0.1mg0.1mg£449.00
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Further Information

Additional Names:
Hairy and enhancer of split 5, hes family bHLH transcription factor 5, bHLHb38
Application Note:
HES5 antibody can be used for detection of HES5 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Background:
HES5 is a member of the HES family of transcriptional repressors, which are the mammalian homologs of the Drosophila Hairy and Enhancer of Split and contain basic Helix-loop-helix (bHLH) and Orange domains (1). The HES5 gene is a downstream target of the Notch signaling pathway and its expression is downregulated during human cartilage differentiation (2). HES5 can directly repress transcription of the E3 ligase FBW7-beta, creating a feedback loop that modulates Notch-mediated intestinal and neural stem cell fate decisions (3).
Background References:
  • Katoh M and Katoh M. Identification and characterization of human HES2, HES3, and HES5 genes in silico. Int. J. Oncol. 2004; 25:529-34.
  • Karlsson C, Jonsson M, Asp J, et al. Notch and HES5 are regulated during human cartilage differentiation. Cell Tissue Res. 2007; 327:539-51.
  • Sancho R, Blake SM, Tendeng C, et al. Fbw7 repression by hes5 creates a feedback loop that modulates Notch-mediated intestinal and neural stem cell fate decisions. PLoS Biol. 2013; 11:e1001586.
Buffer:
HES5 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
HES5 antibody was raised against a 19 amino acid peptide near the amino terminus of human HES5.

The immunogen is located within the first 50 amino acids of HES5.
NCBI Gene ID #:
388585
NCBI Official Name:
hes family bHLH transcription factor
NCBI Official Symbol:
HES5
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 18 kDa

Observed: 17 kDa
Protein Accession #:
NP_001010926
Protein GI Number:
58219048
Purification:
HES5 antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
HES5 antibody is human, mouse and rat reactive. At least two isoforms of HES5 are known to exist; this antibody will detect both isoforms.
Swissprot #:
Q5TA89
User NOte:
Optimal dilutions for each application to be determined by the researcher.