Further Information
PPARGC1A Antibody: LEM6, PGC1, PGC1A, PGC-1v, PPARGC1, PGC-1(alpha), LEM6, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, Ligand effect modulator 6, PGC-1-alpha
WB: 2 μg/mL; IHC: 1-2 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse and rat samples; Immunofluorescence in human samples. All other applications and species not yet tested.
The peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A), also known as LEM-6, is a transcriptional coactivator that regulates the genes involved in energy metabolism (1). PPARGC1A interacts with PPARgamma, which permits the interaction of PPARGC1A with multiple transcription factors. PPARGC1A can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination (2). PPARGC1A may be also involved in the development of obesity (3).
- Tsuemi T and La Spada AR. PGC-1a at the intersection of bioenergetics regulation and neuron function: from Huntington's disease to Parkinson's disease and beyond. Prog. Neurobiol. 2012; 97:142-51.
- Kang C and Li Ji L. Role of PGC-1a signaling in skeletal muscle health and disease. Ann. NY Acad. Sci. 2012; 1271:110-7.
- Liu C and Lin JD. PGC-1 coactivators in the control of energy metabolism. Acta Biochim. Biophys. Sin. 2011; 43:248-57.
PPARGC1A antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (94%)
PPARGC1A antibody was raised against a 17 amino acid peptide near the amino terminus of human PPARGC1A.
The immunogen is located within amino acids 200 - 250 of PPARGC1A.
10891
peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
PPARGC1A
homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 91kD
Observed: 100 kD
NP_037393
7019499
PPARGC1A antibody is affinity chromatography purified via peptide column.
Obesity
PPARGC1A antibody is human, mouse and rat reactive. At least three isoforms of PPARGC1A are known to exist; this antibody will detect all three isoforms.
Q9UBK2
Optimal dilutions for each application to be determined by the researcher.